Pubblicazioni Scientifiche

Dr. Francesco Fiorentino, Phd

Biologo Molecolare e Manager che opera nel settore della Genetica della Riproduzione da oltre 25 anni, sia nella gestione di laboratori di genetica che nell’ambito della ricerca scientifica.


Elenco degli articoli scientifici pubblicati su riviste internazionali

  1. Fiorentino F, Magli MC, Podini D, Ferraretti AP, Nuccitelli A, Vitale N, Baldi M, Gianaroli L. The minisequencing method: an alternative strategy for preimplantation genetic diagnosis of single gene disorders. Mol Hum Reprod. 2003 Jul;9(7):399-410.
  2. Gianaroli L, Magli MC, Fiorentino F, Baldi M, Ferraretti AP. Clinical value of preimplantation genetic diagnosis. Placenta. 2003 Oct;24 Suppl B:S77-83
  3. M Iacobelli, E Greco, L Rienzi, F Ubaldi, D Podini, A Nuccitelli, Jan Tesarik, M Baldi and F Fiorentino Preimplantation genetic diagnosis for Charcot–Marie–Tooth type X  Reproductive BioMedicine Online 2003 Vol. 7, No. 5 558–562
  4. Biroccio A., Fiorentino F., et al. (2004) “Glutathione Depletion Induced by c-Myc Down-regulation Triggers the Apoptotic Pathway Upon Treatment with Alkylating Agents” Neoplasia May-Jun; 6(3):195-206
  5. Fiorentino F., Biricik A., Karadayi H.; Berkil H., Karlıkaya G., Sertyel S., Podini D., Baldi M., Magli MC., Gianaroli L.  and Kahraman S. (2004) Development and clinical application of a strategy for preimplantation genetic diagnosis of single gene disorders combined with HLA matching. Mol. Hum. Reprod. 10: 445-460.
  6. Fiorentino F., Kahraman S., Karadayi H., Biricik A., Sertyel S., Karlıkaya G., Saglam Y.,  Nuccitelli A. and Baldi M. (2005) Short tandem repeats haplotyping of the HLA region  in preimplatation HLA matching. Eur J Hum Genet. 13: 953-958.
  7. Guercini F, Pajoncini C, Bard R, Fiorentino F, Bini V, Costantini E, Porena M. Echoguided drug infiltration in chronic prostatitis: results of a multi-centre study. Arch Ital Urol Androl. 2005 Jun;77(2):87-92.
  8. Benkhalifa M, Kasakyan S, Clement P, Baldi M, Tachdjian G, Demirol A, Gurgan T, Fiorentino F, Mohammed M, Qumsiyeh MB. (2005) Array comparative genomic hybridization profiling of first-trimester spontaneous abortions that fail to grow in vitro. Prenat Diagn. Oct;25(10):894-900.
  9. Benassi B, Fanciulli M, Fiorentino F, Porrello A, Chiorino G, Loda M, Zupi G, Biroccio A. c-Myc Phosphorylation Is Required for Cellular Response to Oxidative Stress. Mol Cell. 2006 Feb 17;21(4):509-19.
  10. Fiorentino F., Biricik A., Nuccitelli A., De Palma R., Kahraman S., Iacobelli M., Trengia V., Caserta D., Bonu M.A., Borini A., Baldi M. Strategies and clinical outcome of 250 cycles of preimplantation genetic diagnosis for single gene disorders Hum Reprod (2006) 21: 670-684.
  11. Kahraman S, Findikli N, Karliklaya G, Sertyel S, Karadayi H, Saglam Y, Fiorentino F. Medical and social perspectives of PGD for single gene disorders and human leukocyte antigen typing Reprod Biomed Online. 2007 Feb;14 Suppl 1:104-8.
  12. Fiorentino F, Biricik A, Nuccitelli A, De Palma R, Kahraman S, Sertyel S, Karadayi H, Cottone G, Baldi M, Caserta D, Moscarini M. Rapid protocol for pre-conception genetic diagnosis of single gene mutations by first polar body analysis: a possible solution for the Italian patients Prenatal Diagnosis 2008 28(1):62-64
  13. Harper J, Sermon K, Geraedts J, Vesela K, Harton G, Thornhill A, Pehlivan T, Fiorentino F, Sengupta S, de Die-Smulders C, Magli C, Moutou C, Wilton L What next for preimplantation genetic screening? Hum Reprod. 2008 Mar;23(3):478-80.
  14. Caserta D, Benkhalifa M, Baldi M, Fiorentino F, Qumsiyeh M, Moscarini M. Genome profiling of ovarian adenocarcinomas using pangenomic BACs microarray comparative genomic hybridization. Mol Cytogenet. 2008 May 20;1(1):10.
  15. Van de Velde H, De Rycke M, De Man C, De Hauwere K, Fiorentino F, Kahraman S, Pennings G, Verpoest W, Devroey P, Liebaers I. The experience of two European preimplantation genetic diagnosis centres on human leukocyte antigen typing. Hum Reprod. 2009 Mar;24(3):732-40.
  16. Fusco C, Frattini D, Farnetti E, Nicoli D, Casali B, Fiorentino F, Nuccitelli A, Giustina ED. Hereditary spastic paraplegia and axonal motor neuropathy caused by a novel SPG3A de novo mutation. Brain Dev. 2010 Aug;32(7):592-4.
  17. Joyce Harper, Edith Coonen , Martine De Rycke, , Francesco Fiorentino, Joep Geraedts, Veerle Goossens, Gary Harton, Celine Moutou, Tugce Pehlivan, Pam Renwick, Sioban SenGupta, Joanne Traeger-Synodinos, Katerina Vesela. What next for Preimplantation Genetic Screening (PGS)?  A position statement from the ESHRE PGD Consortium Steering Committee. Hum Reprod 2010;25:821-823.
  18. Fiorentino F, Kokkali G, Biricik A, Stavrou D, Ismailoglu B, De Palma R, Arizzi L, Harton G, Sessa M, Pantos K. PCR-based detection of chromosomal imbalances on embryos: the evolution of PGD for chromosomal translocations. Fertil Steril. 2011 Nov;94(6):2001-11
  19. Harton GL, De Rycke M, Fiorentino F, Moutou C, SenGupta S, Traeger-Synodinos J, Harper JC; European Society for Human Reproduction and Embryology (ESHRE) PGD Consortium. ESHRE PGD consortium best practice guidelines for amplification-based PGD. Hum Reprod 2011;26:33-40.
  20. Fiorentino F, Spizzichino L, Bono S, Biricik A, Kokkali G, Rienzi L, Ubaldi F.M, Iammarrone E, Gordon A, Pantos K. Preimplantation genetic diagnosis for reciprocal and robertsonian translocations using array comparative genomic hybridization. Hum Reprod 2011; 26:1925-35.
  21. Fiorentino F, Caiazzo F, Napolitano S, Spizzichino L, Bono S, Sessa M, Nuccitelli A, Biricik A, Gordon A, Rizzo G, Baldi M. Introducing array comparative genomic hybridization into routine prenatal diagnosis practice: a prospective study on over 1000 consecutive clinical cases. Prenat Diagn. 2011 Dec;31(13):1270-82.
  22. Fiorentino F, Baldi M. Re: Microarray application in prenatal diagnosis: a position statement from the cytogenetics working group of the Italian Society of Human Genetics (SIGU), November 2011. Ultrasound Obstet Gynecol. 2012 May;39(5):601-2
  23. Fiorentino F. Molecular genetic analysis of single cells. Semin Reprod Med. 2012 Aug;30(4):267-82.
  24. Fiorentino F. Array comparative genomic hybridization: its role in preimplantation genetic diagnosis. Curr Opin Obstet Gynecol. 2012 Aug;24(4):203-9.
  25. Fiorentino F, Caiazzo F, Napolitano S, Spizzichino L, Bono S, Sessa M, Nuccitelli A, Biricik A, Gordon A, Rizzo G, Baldi M. Array CGH in routine prenatal diagnosis practice: reply letter. Prenat Diagn. 2012;32:711-712.
  26. Capalbo A, Bono S, Spizzichino L, Biricik A, Baldi M, Colamaria S, Ubaldi FM, Rienzi L, Fiorentino F. Sequential comprehensive chromosome analysis on polar bodies, blastomeres and trophoblast: insights into female meiotic errors and chromosomal segregation in the preimplantation window of embryo development. Hum Reprod. 2013 Feb;28(2):509-18.
  27. Deans Z, Fiorentino F, Biricik A, Traeger-Synodinos J, Moutou C, De Rycke M, Renwick P, Sengupta S, Goossens V, Harton G. The experience of 3 years of external quality assessment of preimplantation genetic diagnosis for cystic fibrosis. Eur J Hum Genet. 2013 Aug;21(8):800-6.
  28. Fiorentino F, Napoletano S, Caiazzo F, Sessa M, Bono S, Spizzichino L, Gordon A, Nuccitelli A, Rizzo G, Baldi M. Chromosomal microarray analysis as a first-line test in pregnancies with a priori low risk for the detection of submicroscopic chromosomal abnormalities. Eur J Hum Genet. 2013 Jul;21(7):725-30.
  29. Capalbo A, Bono S, Spizzichino L, Biricik A, Baldi M, Colamaria S, Ubaldi FM, Rienzi L, Fiorentino F. Reply: Questions about the accuracy of polar body analysis for preimplantation genetic screening. Hum Reprod. 2013 Jun;28(6):1733-6.
  30. Fiorentino F. Re: Use of prenatal chromosomal microarray: prospective cohort study and systematic review and meta-analysis. Ultrasound Obstet Gynecol. 2013 Jun;41(6):608.
  31. Fiorentino F., Biricik A., Bono S., Spizzichino L., Cotroneo E., Cottone G., Kokocinski F., Michel C. Development and validation of a next-generation sequencing (NGS)-based protocol for 24-chromosome aneuploidy screening of embryos. Fertil Steril 2014 101(5):1375-1382.
  32. Greco E, Bono S, Ruberti A, Lobascio AM, Greco P, Biricik A, Spizzichino L, Greco A, Tesarik J, Minasi MG, Fiorentino F. Comparative genomic hybridization selection of blastocysts for repeated implantation failure treatment: a pilot study. Biomed Res Int. 2014;2014:457913.
  33. Fiorentino F, Bono S, Biricik A, Nuccitelli A, Cotroneo E, Cottone G, Kokocinski F, Michel CE, Minasi MG, Greco E. Application of next-generation sequencing technology for comprehensive aneuploidy screening of blastocysts in clinical preimplantation genetic screening cycles. Hum Reprod. 2014 Dec;29(12):2802-13.
  34. Fiorentino F, Bono S, Biricik A, Nuccitelli A, Cotroneo E, Cottone G, Kokocinski F, Michel CE, Minasi MG, Greco E. Reply: Which blastocysts should be considered for genetic screening? Hum Reprod. 2015 Jul;30(7):1744-5.
  35. Greco E, Biricik A, Cotarelo RP, Iammarone E, Rubino P, Tesarik J, Fiorentino F, Minasi MG. Successful implantation and live birth of a healthy boy after triple biopsy and double vitrification of oocyte-embryo-blastocyst. Springerplus. 2015 4:22.
  36. Bono S, Biricik A, Spizzichino L, Nuccitelli A, Minasi MG, Greco E, Spinella F, Fiorentino F. Validation of a semiconductor next-generation sequencing (NGS)-based protocol for preimplantation genetic diagnosis of reciprocal translocation. Pren Diagn 2015 35:938-44.
  37. Greco E, Minasi MG, Fiorentino F. Healthy babies born after intrauterine transfer of mosaic aneuploidy blastocyst. N Engl J Med 2015; 373:2089-90.
  38. Girardet A, Viart V, Plaza S, Daina G, De Rycke M, Des Georges M, Fiorentino F, Harton G, Ishmukhametova A, Navarro J, Raynal C, Renwick P, Saguet F, Schwarz M, SenGupta S, Tzetis M, Roux AF, Claustres M. The improvement of the best practice guidelines for preimplantation genetic diagnosis of cystic fibrosis: toward an international consensus. Eur J Hum Genet. Eur J Hum Genet. 2016 Apr;24(4):469-78.
  39. Fiorentino F, Bono S, Pizzuti F, Mariano M, Polverari A, Duca S, Sessa M, Baldi M, Diano L, Spinella F. The importance of determining the limit of detection of non-invasive prenatal testing methods. Prenat Diagn. 2016 Apr;36:304-11.
  40. Greco E, Litwicka K, Arrivi C, Varricchio MT, Caragia A, Greco A, Minasi MG, Fiorentino F. The endometrial preparation for frozen-thawed euploid blastocyst transfer: a prospective randomized trial comparing clinical results from natural modified cycle and exogenous hormone stimulation with GnRH agonist. J Assist Reprod Genet. 2016 Jul;33(7):873-84.
  41. Sermon K, Capalbo A, Cohen J, Coonen E, De Rycke M, De Vos A, Delhanty J, Fiorentino F, Gleicher N, Griesinger G, Grifo J, Handyside A, Harper J, Kokkali G, Mastenbroek S, Meldrum D, Meseguer M, Montag M, Munné S, Rienzi L, Rubio C, Scott K, Scott R, Simon C, Swain J, Treff N, Ubaldi F, Vassena R, Vermeesch JR, Verpoest W, Wells D, Geraedts J. The why, the how and the when of PGS 2.0: current practices and expert opinions of fertility specialists, molecular biologists, and embryologists. Mol Hum Reprod 2016;22:845-57.
  42. Minasi MG, Colasante A, Riccio T, Ruberti A, Casciani V, Scarselli F, Spinella F, Fiorentino F, Varricchio MT, Greco E. Correlation between aneuploidy, standard morphology evaluation and morphokinetic development in 1730 biopsied blastocysts: a consecutive case series study. Hum Reprod 2016 31:2245-2254.
  43. Fiorentino F, Bono S, Pizzuti F, Mariano M, Polverari A, Duca S, Sessa M, Baldi M, Diano L, Spinella F. Response to “The importance of determining the limit of detection of non-invasive prenatal testing methods”. Prenat Diagn. 2016 36:898-899.
  44. Sermon KD, Spits C, Mertzanidou A, Vermeesch JR, Fiorentino F. Detecting mosaicism in trophectoderm biopsies. Hum Reprod. 2016 22;7:77378-77388.
  45. Griffin DK, Fishel S, Gordon T, Yaron Y, Grifo J, Hourvitz A, Rechitsky S, Elson J, Blazek J, Fiorentino F, Treff N, Munne S, Leong M, Schmutzler A, Vereczkey A, Ghobara T, Nánássy L, Large M, Hamamah S, Anderson R, Gianaroli L, Wells D. Continuing to deliver: the evidence base for pre-implantation genetic screening. BMJ. 2017 Feb 14;356:j752.
  46. Fiorentino F, Bono S, Pizzuti F, Duca S, Polverari A, Faieta M, Baldi M, Diano L, Spinella F. The clinical utility of genome-wide non invasive prenatal screening. Pren Diagn 2017;37:593-601.
  47. Harton GL, Cinnioglu C, Fiorentino F. Current experience concerning mosaic embryos diagnosed during preimplantation genetic screening. Fertil Steril. 2017 May;107(5):1113-1119.
  48. Minasi MG, Fiorentino F, Ruberti A, Biricik A, Cursio E, Cotroneo E, Varricchio MT, Surdo M, Spinella F, Greco E. Genetic diseases and aneuploidies can be detected with a single blastocyst biopsy: a clinical successful approach. Hum Reprod 2017; 12:1-8
  49. La Marca A, Minasi MG, Sighinolfi G, Greco P, Argento C, Grisendi V, Fiorentino F, Greco E. Female age, serum antimüllerian hormone level, and number of oocytes affect the rate and number of euploid blastocysts in in vitro fertilization/intracytoplasmic sperm injection cycles. Fertil Steril 2017;108:777-783.
  50. Fiorentino F, Bono S, Pizzuti F, Duca S, Polverari A, Faieta M, Baldi M, Diano L, Spinella F. Author’s reply to Grati and Benn. Prenat Diagn 2017;37:1053-1054.
  51. Spinella F, Fiorentino F, Biricik A, Bono S, Ruberti A, Cotroneo E, Baldi M, Cursio E, Minasi MG, Greco E. The extent of chromosomal mosaicism influences the clinical outcome of in vitro fertilization treatments. Fertil Steril 2018;109:77-83.
  52. Rosenwaks Z, Handyside AH, Fiorentino F, Gleicher N, Paulson RJ, Schattman GL, Scott RT Jr, Summers MC, Treff NR, Xu K. The pros and cons of preimplantation genetic testing for aneuploidy: clinical and laboratory perspectives. Fertil Steril. 2018 Aug;110(3):353-361.
  53. Munné S, Spinella F, Grifo J, Zhang J, Beltran MP, Fragouli E, Fiorentino F. Clinical outcomes after the transfer of blastocysts characterized as mosaic by high resolution Next Generation Sequencing- further insights. Eur J Med Genet. 2020 Feb;63(2):103741.
  54. Allegretti M, Cottone G, Carboni F, Cotroneo E, Casini B, Giordani E, Amoreo CA, Buglioni S, Diodoro M, Pescarmona E, Zazza S, Federici O, Zeuli M, Conti L, Cigliana G, Fiorentino F, Valle M, Giacomini P, Spinella F. Cross-sectional analysis of circulating tumor DNA in primary colorectal cancer at surgery and during post-surgery follow-up by liquid biopsy. J Exp Clin Cancer Res. 2020 Apr 20;39(1):69.
  55. La Marca A, Capuzzo M, Sacchi S, Imbrogno MG, Spinella F, Varricchio MT, Minasi MG, Greco P, Fiorentino F, Greco E. Comparison of euploidy rates of blastocysts in women treated with progestins or GnRH antagonist to prevent the luteinizing hormone surge during ovarian stimulation. Hum Reprod. 2020 Jun 1;35(6):1325-1331.
  56. Dinami R, Porru M, Amoreo CA, Sperduti I, Mottolese M, Buglioni S, Marinelli D, Maugeri-Saccà M, Sacconi A, Blandino G, Leonetti C, Di Rocco G, Verdina A, Spinella F, Fiorentino F, Ciliberto G, Biroccio A, Zizza P. TRF2 and VEGF-A: an unknown relationship with prognostic impact on survival of colorectal cancer patients. J Exp Clin Cancer Res. 2020 Jun 15;39(1):111.
  57. LA Marca A, Capuzzo M, Imbrogno MG, Donno V, Spedicato GA, Sacchi S, Minasi MG, Spinella F, Greco P, Fiorentino F, Greco E. The complex relationship between female age and embryo euploidy. Minerva Obstet Gynecol. 2021 Feb;73(1):103-110.
  58. Biricik A, Cotroneo E, Minasi MG, Greco PF, Bono S, Surdo M, Lecciso F, Sessa M, Fiorentino F, Spinella F, Greco E. Cross-Validation of Next-Generation Sequencing Technologies for Diagnosis of Chromosomal Mosaicism and Segmental Aneuploidies in Preimplantation Embryos Model. Life (Basel). 2021 Apr 12;11(4):340.
  59. Ganci F, Allegretti M, Manciocco V, Fiorentino F, Sanguineti G, Giacomini P, Pellini R, Spinella F, Blandino G. Two distinct TP53 mutations in HNSCC primary tumor: Only one circulates in the blood. Oral Oncol. 2021 Apr;115:105096.
  60. Viotti M, Victor AR, Barnes FL, Zouves CG, Besser AG, Grifo JA, Cheng EH, Lee MS, Horcajadas JA, Corti L, Fiorentino F, Spinella F, Minasi MG, Greco E, Munné S. Using outcome data from one thousand mosaic embryo transfers to formulate an embryo ranking system for clinical use. Fertil Steril. 2021 May;115(5):1212-1224.
  61. Oliveri S, Cincidda C, Ongaro G, Cutica I, Gorini A, Spinella F, Fiorentino F, Baldi M, Pravettoni G. What people really change after genetic testing (GT) performed in private labs: results from an Italian study. Eur J Hum Genet. 2022 Jan;30(1):62-72.
  62. La Marca A, Capuzzo M, Longo M, Imbrogno MG, Spedicato GA, Fiorentino F, Spinella F, Greco P, Minasi MG, Greco E. The number and rate of euploid blastocysts in women undergoing IVF/ICSI cycles are strongly dependent on ovarian reserve and female age. Hum Reprod. 2022 Sep 30;37(10):2392-2401.
  63. De Rycke M, Capalbo A, Coonen E, Coticchio G, Fiorentino F, Goossens V, Mcheik S, Rubio C, Sermon K, Sfontouris I, Spits C, Vermeesch JR, Vermeulen N, Wells D, Zambelli F, Kakourou G. ESHRE survey results and good practice recommendations on managing chromosomal mosaicism. Hum Reprod Open. 2022 Nov 7;2022(4):hoac044.

Elenco di libri (o capitoli) nazionali ed internazionali

  1. Francesco Fiorentino  PREIMPLANTATION GENETIC DIAGNOSIS, CURRENT STATUS AND FUTURE PROSPECTS (2008) 24^ Recent advances in obstetrics and gynaecology – W. Dunlop, W. Ledger – The Royal Society of Medicine Press.
  2. Francesco Fiorentino LA DIAGNOSI GENETICA PREIMPIANTO: PROBLEMI PRATICI E QUESTIONI APPLICATIVE IN CAMPO MEDICO – Da “Tecnologie riproduttive e tutela della persona” – G. Baldini, M. Soldano – (2008) Firenze University Press.
  3. F. Fiorentino, M. Baldi, M. Benkhalifa, L. Chessa, D. Caserta, M. Moscarini  (2008) THE GENETICS OF POLYCYSTIC OVARIAN SYNDROME DA POLYCYSTIC OVARIAN SYNDROME ed. Gautam N Allahbadia, Rina Agrawal.
  4. Francesco Fiorentino Alan H. Handyside, Mark D. Robinson PREIMPLANTATION GENETIC DIAGNOSIS USING WHOLE GENOME AMPLIFICATION WHOLE GENOME AMPLIFICATION, Chapter 11, edited by S.Huges and R. Lasken, 2006.
  5. F. Fiorentino, M. Baldi, M. Benkhalifa, D. Caserta, M. Moscarini. (2009) Diagnosi Preimpianto – Da “Diagnosi Prenatale”, Carmine Nappi, felice Pretraglia, ed. Poletto
  7. Francesco Fiorentino, Ermanno Greco, Maria Giulia Minasi, Francesca Spinella – (2020) TRANSFER OF MOSAIC EMBRYOS – Da  “Preimplantation Genetic Testing – Recent Advances in Reproductive Medicine”. Taylor & Francis Group.